An improved purification procedure for Leishmania RNA virus (LRV)

نویسندگان

  • Marcos Michel de Souza
  • Livia Regina Manzine
  • Marcos Vinicius G. da Silva
  • Jefferson Bettini
  • Rodrigo Vilares Portugal
  • Angela Kaysel Cruz
  • Eurico Arruda
  • Otavio Henrique Thiemann
چکیده

Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.

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عنوان ژورنال:

دوره 45  شماره 

صفحات  -

تاریخ انتشار 2014